Challenges in Polycaprolactone Nanofibres (PCL) Scaffold’s Strand

Phaos Case Studies

Nanofibre technology is a useful and very recent tool in stem cell biology as the special properties of nanofibres make them suitable for a wide range of biomedical applications. Polymer‐based nanofibres belong to a new class of biomaterials that could be produced using electrospinning technology.

Electrospun nanofibers constitute different depth layers of strand across the surface. Hence, the limited depth of field (DoF) of the optical microscope has been a hurdle to resolve all features completely in a real-time image.

Material Illustrated: Polycaprolactone Nanofibre

The issue is amplified in nanosize imaging where a higher resolution optical lens is used. This leads to receiving limited information on the image for further analysis as the image captured will be less reliable, accurate and consistent.

Material Illustrated: Polycaprolactone Nanofibre

However, with our new technology in our ON200 optical microscopy system, this brings a silver lining to nano-technology based industry.

Our ON200 microscopy system-generated the following results to the Well-Known Research Institute in Singapore.

  1. The average Diameter of Polycaprolactone Nanofibers
  2. Presence of Colony Formation (Cell Charging)
  3. Confluency (Number) of Polycaprolactone Nanofibers

Nanofibre technology not merely bound to the biotechnology industry and the trend is visibly emerging across the various industry for different application purposes.

Click here for ON200 microscope product information.